Extending The Reach Of Amperia™: Simplifying His-tagged Protein Quantification Across Expression And Development Workflows

Sponsored Content by AbselionReviewed by Olivia FrostNov 27 2025

In this interview, News Medical-Life Sciences speaks pinch Dr. Ruizhi Wang, Founder and CEO of Abselion, astir simplifying nan quantification of His-tagged proteins utilizing nan Amperia™ platform. Dr. Wang, discusses really electrochemical sensing, validated reagents, and user-focused workflows are helping researchers move from analyzable protocols to consistent, scalable measurement.

To begin, could you concisely present Abselion and your domiciled successful nan improvement and exertion of nan Amperia™ platform?

I’m nan Founder and CEO of Abselion, a Cambridge-based life sciences exertion institution focused connected simplifying biomolecule quantification. We create applicable analytical devices that thief scientists make reliable information without nan complexity of accepted systems.

Amperia™ is simply a compact benchtop level that uses electrochemical sensing to quantify proteins and viral vectors quickly and reproducibly. It combines tested chemistry, engineered sensors, and intuitive package into a single, integrated workflow. Our extremity is to move analyzable analytical tasks into straightforward measurements that tin beryllium tally straight from crude aliases purified samples.

In my role, I lead Abselion’s wide strategy and merchandise development, moving intimately pinch our R&D, production, and commercialized teams to move caller ideas into dependable products. My inheritance successful physics and biosensors has shaped our attack to innovation. We use engineering precision to biologic systems to create devices that are robust, scalable, and genuinely useful for mundane investigation and development.

His-tagged proteins are wide utilized crossed various look systems, yet quantification remains a challenge. What circumstantial gaps aliases inefficiencies did Abselion purpose to reside pinch nan Tagged Protein Quantification Kit?

Quantifying His-tagged proteins is simply a regular portion of recombinant macromolecule workflows, but obtaining reliable, comparable results crossed constructs and experiments tin still beryllium difficult.

Techniques specified arsenic gels aliases colorimetric assays are useful for speedy checks, but they only connection comparative penetration and tin alteration pinch sample mentation aliases look background. More quantitative approaches, while effective, often trust connected purification aliases multi-step protocols that are difficult to use successful regular research.

The His-tag Protein Quantification Kit was developed to make this measurement much accordant and accessible. It provides a ready-to-use assay for quantitative study straight from crude aliases clarified lysates. The workflow is straightforward and reduces some handling steps and variability betwixt users.

By removing nan applicable barriers to reliable His-tag measurement, nan kit helps researchers attraction connected information mentation alternatively than setup. It enables much assured comparisons crossed constructs, conditions, and stages of development, and supports a much data-driven attack to macromolecule research.

Image Credit: Abselion

Can you explicate really nan Amperia™ platform's redox electrochemical discovery method differs from accepted quantification techniques for illustration ELISA aliases spectrophotometry, particularly successful position of capacity successful crude samples?

Traditional techniques, specified arsenic ELISA aliases spectrophotometry, trust connected optical measurements, which tin beryllium affected by turbidity, color, aliases inheritance absorbance. These are communal issues erstwhile moving pinch crude lysates.

Amperia™ uses a redox-based electrochemical discovery method. Instead of relying connected light, nan level measures an electrical awesome generated by a defined redox guidance connected nan sensor surface, which straight reflects nan magnitude of target bound.

Because Amperia™ does not trust connected optics aliases fluidics, it is little delicate to sample interference and offers faster, much accordant measurements. Sensor handling and readout are automated wrong nan instrument, which improves reproducibility and reduces manual steps that often present variability successful accepted assays.

The caller assay operates utilizing a premix title format. Could you elaborate connected really this creation simplifies workflows and enhances reproducibility for extremity users?

The assay uses a premix title format, wherever nan sample containing His-tagged proteins is mixed pinch a branded discovery reagent anterior to measurement. Both components compete for a fixed number of anti-His binding sites connected nan sensor surface.

When much target macromolecule is present, it occupies much of nan sites, leaving less disposable for nan branded reagent. This results successful a little awesome that is inversely proportional to nan target concentration.

This format eliminates nan request for abstracted binding and washing steps, which simplifies nan workflow and reduces variability. Because nan target and discovery reagent interact nether nan aforesaid conditions, nan assay remains robust moreover pinch analyzable samples, specified arsenic crude lysates. It delivers accordant assay behaviour crossed users, instruments, and look systems.

The kit incorporates anti-His antibodies supplied by GenScript. How does their integration onto nan sensor strips amended assay consistency and trim manual intervention?

The His-tag Protein Quantification Kit was developed successful collaboration pinch GenScript, whose THE™ His Tag Monoclonal Antibody exertion is known for its precocious affinity and reliable nickname of His-tags crossed constructs and systems. By integrating this antibody straight onto Amperia’s sensor strips, we guarantee accordant seizure capacity while maintaining wide compatibility pinch communal tag configurations.

Each portion is pre-coated nether controlled manufacturing conditions. This removes nan request for users to grip aliases optimize reagents, importantly reducing assay-to-assay variability and improving reproducibility betwixt users and sites.

How does nan Amperia™ level support scalability from early look screening done to downstream development, and what benefits does this bring to bioprocessing teams?

Amperia™ brings a accordant attack to biomolecule quantification crossed nan improvement pipeline, from early look screening to purification and process optimization. While individual assays whitethorn alteration successful design, they travel nan aforesaid operating rule and guided workflow. This makes it easy to make comparable, high-quality information crossed aggregate applications, including His-tag proteins, antibodies, and AAVs.

Scalability successful this discourse is much astir elasticity than throughput. Each automated tally is speedy and self-contained. Users tin tally a fewer samples aliases dozens, depending connected task needs. This makes Amperia™ well-suited for early screening arsenic good arsenic in-depth process studies, without requiring caller methods aliases retraining.

Image Credit: Abselion

In summation to nan afloat quantification kit, Abselion has introduced a standalone sensor portion pack. What needs aliases personification feedback led to this modular offering, and really do you spot it being utilized successful divers laboratory settings?

The thought came straight from personification feedback. Many researchers wanted much flexibility, either to widen existing assays aliases to research civilization applications utilizing their ain reagents. By offering standalone sensor portion packs, we alteration users to prime nan configuration that champion fits their workflow.

Different sensor types are available, depending connected nan application, including pre-coated antibody strips for quantification kits and functionalized surfaces, for illustration streptavidin, for civilization assay development. All sensor strips are manufactured and quality-controlled to nan aforesaid modular arsenic our kits.

Finally, really do you spot this attack to His-tagged macromolecule quantification shaping early trends successful macromolecule analytics and bioassay development?

We are seeing increasing request for devices that simplify macromolecule analytics without compromising connected information quality. Platforms for illustration Amperia™ support that displacement by reducing complexity, expanding reproducibility, and enabling faster decisions based connected robust data.

As nan section evolves, I expect much integration of validated reagents, automation, and thoughtful engineering. The early of macromolecule quantification is not conscionable astir improving sensitivity aliases specificity. It is astir making high-quality measurement accessible, efficient, and scalable crossed nan full R&D pipeline.

About Dr. Ruizhi Wang

Dr. Ruizhi Wang is nan laminitis and Chief Executive Officer of Abselion, a Cambridge (UK)-based institution processing applicable analytical solutions for biologics and compartment and cistron therapy. He leads nan company’s wide strategy, planning, and operations, pinch a attraction connected making precocious biomolecule analytics much accessible to researchers and manufacture users.

Ruizhi is an award-winning intelligence and entrepreneur, recognised by nan Royal Society of Chemistry, Merck Group, and Cambridge University Entrepreneurs. He is besides a Royal Academy of Engineering Enterprise Fellow and a erstwhile clever clever of nan German Academic Merit Foundation.

He holds a PhD successful Physics from nan University of Cambridge and MSc/BSc degrees successful interdisciplinary sciences from ETH Zurich. He has co-authored much than 20 technological publications pinch complete 700 citations.

Education & Qualifications: PhD, University of Cambridge; MSc/BSc, ETH Zurich.
Awards & Fellowships: Royal Academy of Engineering Enterprise Fellow; Royal Society of Chemistry Emerging Technologies Award; Merck Group Innovation Award; Cambridge University Entrepreneurs Award; German Academic Merit Foundation Scholarship.
Professional Memberships: Royal Academy of Engineering Enterprise Hub; Royal Society of Chemistry.
Areas of Expertise: Biosensors, electrochemistry, biomolecule analytics, process development, biologics and cistron therapy workflows, exertion translation, and entrepreneurship

About Abselion

Abselion was founded successful 2018, astatine that clip nether nan sanction HexagonFab, successful a mini area of a laboratory astatine nan University of Cambridge.

We group retired pinch nan extremity to make macromolecule investigation simpler. Scientists should beryllium capable to prosecute their passion for find and innovation, alternatively than walk their valuable clip connected tedious, manual tasks. With RED, we had entree to nan perfect exertion to create this product.

A merchandise that is truthful compact that it could fresh connected each bench, and truthful affordable that it is accessible to everyone. Over nan years, we person designed, built, and tested our first product, Amperia™, and we’re proud to present it to nan world.​